Journal: Nature Communications

Article Title: Inhibiting WNT and NOTCH in renal cancer stem cells and the implications for human patients

doi: 10.1038/s41467-020-14700-7

Figure Lengend Snippet: a , b Treatment schemes and quantifications of tumor volumes of PDX4 and PDX1 tumors ( n = 3 tumors in each group) treated with 100 mg/kg ICG-001 (green diamond), 10 mg/kg DAPT (blue squares), combination (black diamond, PDX1 only) or vehicle (red circles) every three days. Data are shown as mean, error bars represent s.d., p -values: *<0.05, *<0.01, ***<0.001 by two-way ANOVA with Tukey post-test (two-sided). c , d HE and Ki-67 staining of representative sections of tumors of PDX1 and PDX4 (scale bars, 50 µm). e , f Immunofluorescence of β-CATENIN (scale bars, 50 µm) and in situ hybridization for AXIN2 (scale bars 100 µm) in PDX4 and PDX1 tumors in mice treated with 100 mg/kg ICG-001. g , h Immunofluorescence of NOTCH1 and JAG1 in PDX4 and PDX1 tumors in mice treated with 10 mg/kg DAPT (scale bars, 50 µm).

Article Snippet: Section were incubated overnight at 4 °C with antibodies against CA9 (1:200, Abcam), CD10 (1:30, Dako), CD44 (1:400, BD biosciences), CXCR4 (1:200, Abcam), MET (1:50, Cell Signaling Technology), VCAM1 (1:250, Abcam), E-Cadherin (1:1000, BD biosciences), Epcam (1:500, Abcam), Ki-67 (1:200, Thermo Fisher), or JAG1 (1:200, Atlas Antibodies) primary antibodies and Alexa-conjugated secondary antibodies (1:200, Dianova).

Techniques: Staining, Immunofluorescence, In Situ Hybridization

Journal: Experimental and therapeutic medicine

Article Title: Study of dietary‑induced progression of psoriasis‑like mice based on gut macrophage polarization.

doi: 10.3892/etm.2023.11976

Figure Lengend Snippet: Figure 5. Protein expressions of Jagged1/Notch1/HES5 and TLR‑2/IκB‑α/NF‑κB p65 signaling pathway on skin lesions of imiquimod‑induced psoriasis model. (A and a) Western blot analysis of Notch signaling and NRP1 in skin lesions. The mammalian Notch signaling pathway consists of four Notch receptors (Notch 1, 2, 3, and 4) and five Notch ligands (Jagged 1, and 2, and Delta‑like 1, 3 and 4). HES5 is an important downstream target gene of Notch signaling pathway. NRP1 is involved in keratinocyte hyperproliferation of psoriasis accompanied by inflammation activated angiogenesis. (B and b) Western blot analysis of TLR‑2/IκB‑α/NF‑κB p65 signaling in skin lesions. Values are expressed as mean ± SD (*P<0.05, **P<0.01 vs. the normal control; #P<0.05, ##P<0.01, ###P<0.001 vs. the psoriasis group) and were statistically analyzed with one‑way ANOVA. NC, normal control; P, psoriasis; P + SF, psoriasis + SF; SF, stimulating food; p‑, phosphorylated.

Article Snippet: Proteins were transferred onto a PVDF membrane (MilliporeSigma), and then incubated with primary antibodies specific for NF‐κB p65 (cat. no. 6956), phospho‐NF‐κB p65 (cat. no. 3033), Toll‐like receptor‐2 (cat. no. 2229S), IKBα (cat. no. 9242), Jagged‐1 (cat. no. 70109s), Notch‐1 (cat. no. 3608s; all CST; 1:1,000), Hes‐5 (cat. no. ab25374; Abcam; 1:1,000) and anti‐β‐actin (cat. no. BM0627; Wuhan Boster Biological Technology, Ltd.; 1:2,000) overnight at 4 ̊C and then followed by incubation with horseradish peroxidase‐conjugated secondary anti‐mouse or anti‐rabbit IgG (cat. nos.

Techniques: Western Blot, Control